![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0006.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Po, Bv, RM, ShSpecies Glossary |
| Applications | WB, IHC, IP |
| Clone | GT217 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary. |
| Predicted Species | Porcine (99%), Bovine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CFL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NBP3-13520)Find related products by research area.
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ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
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| Gene Symbol | CFL1 |
![Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunohistochemistry-Paraffin-NBP3-13520-img0003.jpg "Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0004.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0005.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520] - Immunoprecipitation of Cofilin protein from 293T whole cell extracts using 5 ug of Cofilin antibody [GT217] (NBP3-13520). Western blot analysis was performed using Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunoprecipitation-NBP3-13520-img0002.jpg "Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520] - Immunoprecipitation of Cofilin protein from 293T whole cell extracts using 5 ug of Cofilin antibody [GT217] (NBP3-13520). Western blot analysis was performed using Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500. EasyBlot anti-Mouse IgG was used as a secondary reagent.")
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-910202420134075.jpg "Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cofilin stained by Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-91020242075996.jpg "Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cofilin stained by Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500.Blue: Fluoroshield with DAPI .")
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