Cofilin Antibody (567) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828] - HBL435 xenograft. Cofilin 1 antibody [567] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Cofilin Antibody (567) [NBP2-42828] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:300.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM, Sh
• Applications: WB, ICC/IF, IHC, KO
• Clone: 567
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cofilin Antibody (567) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Cofilin Antibody (567) - Azide and BSA Free (NBP2-42828) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.
• Predicted Species: Porcine (99%), Bovine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFL1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Cofilin Antibody (567) - Azide and BSA Free

• 18 kDa phosphoprotein
• CFL
• CFL1
• cofilin 1 (non-muscle)
• cofilin
• Cofilin, non-muscle isoform
• cofilin-1
• HEL-S-15
• p18

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFL1