CIB2 Antibody

Images

 
Western Blot: CIB2 Antibody [H00010518-A01] - Detection against Immunogen (37 KDa).
Chromatin Immunoprecipitation: CIB2 Antibody [H00010518-A01] - Analysis of CIB2 in 5dpf zebrafish retina using anti-CIB2 antibody (red) and DAPI (blue). Image from verified customer review.

Product Details

Summary
Reactivity Hu, ZeSpecies Glossary
Applications WB, ChIP, ELISA
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CIB2 Antibody Summary

Immunogen
CIB2 (NP_006374, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag.MGNKQTIFTEEQLDNYQDCTFFNKKDILKLHSRFYELAPNLVPMDYRKSPIVHVPMSLIIQMPELRENPFKERIVAAFSEDGEGNLTFNDFVDMFSVLC
Specificity
CIB2 - calcium and integrin binding family member 2
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
CIB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • ELISA
Application Notes
The quality control of this antibody is limited to Western blot on the immunizing protein. It has also been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500
Reviewed Applications
Read 1 Review rated 4
using
H00010518-A01 in the following applications:

Publications
Read Publications using H00010518-A01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol
Preservative
No Preservative
Purity
Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CIB2 Antibody

  • 2810434I23Rik
  • calcium and integrin binding family member 2
  • DNA-dependent protein kinase catalytic subunit-interacting protein 2
  • Kinase-interacting protein 2
  • KIP 2
  • KIP2calcium and integrin-binding family member 2

Background

The amino acid sequence the protein encoded by this gene is similar to that of KIP/CIB, calcineurin B, and calmodulin. This suggests that the encoded protein may be a Ca2+-binding regulatory protein that interacts with DNA-dependent protein kinase catalytic subunit (DNA-PKcs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIB2 Antibody (H00010518-A01)(2)

Review for CIB2 Antibody (H00010518-A01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using H00010518-A01:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CIB2 H00010518-A01
Enlarge
reviewed by:
Anonymous
IF Other 04/09/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRetina
SpeciesOther

Blocking

Blocking DetailsPBSTx + 2.5% BSA + 2.5% FCS at room temperature for at least 30 min

Primary Anitbody

Dilution Ratioprimary antibody was diluted 1:50 in blocking solution and incubation was done overnight at 4C with rocking

Secondary Antibody

Secondary Descriptiondonkey-anti mouse Alexa Fluor 594
Secondary Manufacturer Cat#A-21203
Secondary Concentration1:200

Details

Detection NotesConfocal microscope, Zeiss 510, Z-stack. Negative control was done in the absence of primary antibody.
Fixation DetailsFish were dehydrated with increasing concentrations of MeOH in PBSTx (PBS 0.1% Triton X-100).
Wash DescriptionThree washes were done with PBSTx for 10 min at room temperature with rocking.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for CIB2 Antibody (H00010518-A01) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CIB2 Antibody (H00010518-A01)

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Diseases for CIB2 Antibody (H00010518-A01)

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Pathways for CIB2 Antibody (H00010518-A01)

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Blogs on CIB2

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Recent Reviews

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5
0
4
1
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0
2
0
1
0

Anonymous
04/09/2016
Application: IF
Species: Other

Bioinformatics

Gene Symbol CIB2
Entrez
OMIM
Uniprot