CHSY1 Antibody

Western Blot: CHSY1 Antibody [NBP2-15899] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Paraffin: CHSY1 Antibody [NBP2-15899] - ES2 xenograft, using CHSY1 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Discontinued

CHSY1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CHSY1. The exact sequence is proprietary.
• Localization: Golgi apparatus; Golgi stack membrane
• Predicted Species: Mouse (96%), Rat (95%), Xenopus (91%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHSY1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus tropicalis (91%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CHSY1 Antibody

• Chondroitin glucuronyltransferase 1
• chondroitin glucuronyltransferase II
• chondroitin sulfate synthase 1
• Chondroitin synthase 1
• CHSY
• ChSy-1
• CSS1DKFZp434M032
• EC 2.4.1.175
• EC 2.4.1.226
• FLJ58581
• Glucuronosyl-N-acetylgalactosaminyl-proteoglycan4-beta-N-acetylgalactosaminyltransferase 1
• KIAA0990carbohydrate (chondroitin) synthase 1
• N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1
• N-acetylgalactosaminyltransferase 1
• N-acetylgalactosaminyltransferase II

Background

CHSY1 synthesizes chondroitin sulfate, a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to a wide range of core protein families and regulate many biologic processes, including cell proliferation and recognition, extracellular matrix deposition, and morphogenesis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CHSY1
• Uniprot:
  • Q86X52(Human)