CHRNB3 Antibody

Western Blot: CHRNB3 Antibody [NB100-94886] - Analysis of CHRNB3 using this antibody at 1 ug/ml in Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CHRNB3 Antibody Summary

• Description: Novus Biologicals Goat CHRNB3 Antibody (NB100-94886) is a polyclonal antibody validated for use in WB and ELISA. Anti-CHRNB3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SRHVKKEHFISQ corresponding to internal region according to NP_000740.1.
• Epitope: C-SRHVKKEHFISQ
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHRNB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human brain (cerebellum) lysates (calculated MW of 52.7 kDa band according to NP_000740.1).
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHRNB3 Antibody

• acetylcholine receptor, neuronal nicotinic, beta-3 subunit
• cholinergic receptor, nicotinic, beta 3
• cholinergic receptor, nicotinic, beta polypeptide 3
• CHRNB3
• NAChRB3
• neuronal acetylcholine receptor subunit beta-3
• Nicotinic Acetylcholine R beta 3

Background

CHRNB3 is associated with subjective responses to tobacco. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator). Observational study and genome-wide association study of gene-disease association. (HuGE Navigator). Observational study of gene-disease association. (HuGE Navigator). absence of differences in the pharmacological profile of nicotinic receptor alpha3beta4 argues against role for incorporated beta3 subunit in formation of agonist binding sites while changes in channel kinetics suggest important effect on receptor gating

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHRNB3 Antibody (NB100-94886)(1)

Publication using NB100-94886

• Zeiger J, Haberstick BC, Schlaepfer I et al. The neuronal nicotinic receptor subunit genes (CHRNA6 and CHRNB3) are associated with subjective responses to tobacco. Hum Mol Genet 2007-11-30 [PMID: 18055561]

Bioinformatics

• Gene Symbol: CHRNB3
• Entrez:
  • 1142(Human)
  • 108043(Mouse)
  • 171131(Rat)
• Uniprot:
  • Q05901(Human)