Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0 to 50 ng/mL |
| Sensitivity |
0.25 ng/mL |
| Inter-Assay |
CV% < 3.13% |
| Intra-Assay |
CV% < 3.50% |
| Spike Recovery |
96.70% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CGB3 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Free beta-CG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of free beta-chorionic gonadotropin. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Enzyme Conjugate Reagent
- Murine Monoclonal Anti-free-beta-hCG-coated microtiter wells.
- Set of Reference Standards: 0, 2.5, 5, 10, 25, and 50 ng/ml, lyophilized
- Stop Solution (1N HCl)
- TMB Reagent (One-Step)
- Zero Buffer (Sample diluent)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)
Background
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (KA0210). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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