![Immunohistochemistry-Paraffin: Chorionic Gonadotropin beta Chain (HCG beta) Antibody (SPM529) [NBP2-34369] - Formalin-fixed paraffin-embedded human placenta stained with hCG beta Monoclonal Antibody (SPM529).](http://images.novusbio.com/fullsize2/Chorionic-Gonadotropin-beta-Chain-(HCG-beta)-Antibody-(SPM529)-Immunohistochemistry-Paraffin-NBP2-34369-img0001.jpg "Immunohistochemistry-Paraffin: Chorionic Gonadotropin beta Chain (HCG beta) Antibody (SPM529) [NBP2-34369] - Formalin-fixed paraffin-embedded human placenta stained with hCG beta Monoclonal Antibody (SPM529).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IHC-Fr, IHC-P |
| Clone | SPM529 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant hCG beta protein |
| Localization | Cytoplasmic, secreted |
| Marker | Pregnancy & Choriocarcinoma Marker |
| Specificity | This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the ? and ? subunits. The ? subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CGB3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA (For coating, order NBP2-34416 (BSA free version of NBP2-34369)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-34416AF350 | |
| Alexa Fluor 405 | NBP2-34416AF405 | |
| Alexa Fluor 488 | NBP2-34416AF488 | |
| Alexa Fluor 532 | NBP2-34416AF532 | |
| Alexa Fluor 594 | NBP2-34416AF594 | |
| Alexa Fluor 647 | NBP2-34416AF647 | |
| Alexa Fluor 700 | NBP2-34416AF700 | |
| Alexa Fluor 750 | NBP2-34416AF750 | |
| Biotin | NBP2-34416B | |
| DyLight 350 | NBP2-34416UV | |
| DyLight 405 | NBP2-34416V | |
| DyLight 488 | NBP2-34416G | |
| DyLight 550 | NBP2-34416R | |
| DyLight 594 | NBP2-34416DL594 | |
| DyLight 650 | NBP2-34416C | |
| DyLight 680 | NBP2-34416FR | |
| DyLight 755 | NBP2-34416IR | |
| FITC | NBP2-34416F | |
| HRP | NBP2-34416H | |
| Janelia Fluor 549 | NBP2-34416JF549 | |
| Janelia Fluor 646 | NBP2-34416JF646 |
Diseases for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (NBP2-34369)Discover more about diseases related to Chorionic Gonadotropin beta Chain (hCG beta) Antibody (NBP2-34369).
| Pathways for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (NBP2-34369)View related products by pathway.
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![Immunocytochemistry/Immunofluorescence: Chromogranin B Antibody [NBP1-80781] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Chromogranin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80781-img0005.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80781] - Staining of human liver.](http://images.novusbio.com/fullsize2/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80781-img0012.jpg)
![Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80781] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize2/Chromogranin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-80781-img0006.jpg)
![Immunohistochemistry-Paraffin: LH beta Antibody (LHb/1214) [NBP2-45130] - Formalin-fixed, paraffin-embedded human Pituitary stained with LH-beta Monoclonal Antibody (LHb/1214).](http://images.novusbio.com/fullsize2/LH-beta-Antibody-(LHb-1214)-Immunohistochemistry-Paraffin-NBP2-45130-img0002.jpg)
![Immunohistochemistry-Paraffin: LH beta Antibody (LHb/1214) [NBP2-45130] - Formalin-fixed, paraffin-embedded human Pituitary stained with LH-beta Monoclonal Antibody (LHb/1214).](http://images.novusbio.com/fullsize2/LH-beta-Antibody-(LHb-1214)-Immunohistochemistry-Paraffin-NBP2-45130-img0001.jpg)
![SDS-Page: LH beta Antibody (LHb/1214) [NBP2-45130]](http://images.novusbio.com/fullsize2/LH-beta-Antibody-(LHb-1214)-SDS-Page-NBP2-45130-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Staining FSH in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hCG Alpha (Intact) Antibody (AH33-72)-Immunocytochemistry-Immunofluorescence-NBP2-67282-img0004.jpg)
![Immunohistochemistry-Paraffin: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Analysis of paraffin-embedded mouse placenta tissue using anti-FSH antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/hCG-Alpha-(Intact)-Antibody-(AH33-72)-Immunohistochemistry-Paraffin-NBP2-67282-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Staining FSH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/hCG-Alpha-(Intact)-Antibody-(AH33-72)-Immunocytochemistry-Immunofluorescence-NBP2-67282-img0001.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis using Anti-PLOD1 antibody NBP2-38770 (A) shows similar pattern to independent antibody NBP2-31885 (B).](http://images.novusbio.com/fullsize2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0004.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/PLOD1 Antibody-Western Blot-NBP2-38770-img0005.jpg)
![Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.](http://images.novusbio.com/fullsize2/Chromogranin-A-Antibody-Immunohistochemistry-Paraffin-NB120-15160-img0003.jpg)
![Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).](http://images.novusbio.com/fullsize2/Chromogranin-A-Antibody-Immunohistochemistry-NB120-15160-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0006.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize2/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize2/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0001.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining in human bone marrow and cerebral cortex tissues using anti-CTSG antibody. Corresponding CTSG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Cathepsin G Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0003.jpg)
![Western Blot: CGB2 Antibody (4F8) [H00114336-M02] - detection against Immunogen (31.9 KDa).](http://images.novusbio.com/fullsize2/CGB2-Antibody-(4F8)-Western-Blot-H00114336-M02-img0001.jpg)
![Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.](http://images.novusbio.com/fullsize/GBA3 Antibody (1F1)-Western Blot-NBP2-00521-img0020.jpg)
![Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (1F1) [NBP2-00521] - GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human prostate tissue using anti-GBA3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize2/Cytosolic-beta-Glucosidase-GBA3-Antibody-(1F1)-Immunohistochemistry-NBP2-00521-img0021.jpg)
![Flow Cytometry: GBA3 Antibody (1F1) [NBP2-00521] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GBA3 Antibody (1F1)-Flow Cytometry-NBP2-00521-img0003.jpg)
![Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Sample Tissue: Human RPMI-8226 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize2/Adenine-Nucleotide-Translocase-1-Antibody-Western-Blot-NBP1-59594-img0005.jpg)
![Immunohistochemistry: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies of pinealocytes and their processes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize2/Adenine-Nucleotide-Translocase-1-Antibody-Immunohistochemistry-NBP1-59594-img0004.jpg)
![Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - RPMI 8226 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/Adenine-Nucleotide-Translocase-1-Antibody-Western-Blot-NBP1-59594-img0002.jpg)
![Western Blot: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/ACAA2-Antibody-(1C10)-Western-Blot-NBP2-46477-img0009.jpg)
![Immunohistochemistry: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/ACAA2-Antibody-(1C10)-Immunohistochemistry-NBP2-46477-img0007.jpg)
![Western Blot: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.](http://images.novusbio.com/fullsize2/ACAA2-Antibody-(1C10)-Western-Blot-NBP2-46477-img0008.jpg)
![Western Blot: Chromogranin C Antibody [NBP2-15895] - A. 50 ug mouse brain lysate/extract 7.5% SDS-PAGE Chromogranin C antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Chromogranin-C-Antibody-Western-Blot-NBP2-15895-img0009.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-15895] - Paraffin-embedded mouse fore brain. Chromogranin C antibody dilution: 1:500.](http://images.novusbio.com/fullsize2/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-15895-img0007.jpg)
![Western Blot: Chromogranin C Antibody [NBP2-15895] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/Chromogranin C Antibody-Western Blot-NBP2-15895-img0001.jpg)
![Western Blot: Pea3 Antibody (3G9-1B9) [H00002118-M01] - Analysis of ETV4 expression in transfected 293T cell line by ETV4 monoclonal antibody (M01), clone 3G9-1B9.Lane 1: ETV4 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/Pea3 Antibody (3G9-1B9)-Western Blot-H00002118-M01-img0004.jpg)
![Sandwich ELISA: Pea3 Antibody (3G9-1B9) [H00002118-M01] - Detection limit for recombinant GST tagged ETV4 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/Pea3-Antibody-(3G9-1B9)-Sandwich-ELISA-H00002118-M01-img0003.jpg)
![Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of (A) Recombinant human Galectin-13 fragment And (B) K562 Cell Lysate using Galeactin-13 MAb (PP13/1161).](http://images.novusbio.com/fullsize2/Galectin-13-Antibody-(PP13-1161)-Western-Blot-NBP2-53210-img0003.jpg)
![Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Spleen stained with Galectin-13MAb (PP13/1161).](http://images.novusbio.com/fullsize2/Galectin-13-Antibody-(PP13-1161)-Immunohistochemistry-Paraffin-NBP2-53210-img0002.jpg)
![Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Placenta stained with Galectin-13 MAb (PP13/1161).](http://images.novusbio.com/fullsize2/Galectin-13-Antibody-(PP13-1161)-Immunohistochemistry-Paraffin-NBP2-53210-img0001.jpg)
![Western Blot: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSH1.](http://images.novusbio.com/fullsize2/Placental Lactogen-CSH1 Antibody (2G4)-Western Blot-NBP2-46376-img0009.jpg)
![Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/Placental-Lactogen-CSH1-Antibody-(2G4)-Immunohistochemistry-NBP2-46376-img0008.jpg)
![Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/Placental-Lactogen-CSH1-Antibody-(2G4)-Immunohistochemistry-NBP2-46376-img0001.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")