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Chorionic Gonadotropin beta Chain (hCG beta) Antibody (SPM105)

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Immunohistochemistry-Paraffin: Chorionic Gonadotropin beta Chain (HCG beta) Antibody (SPM105) [NBP2-34345] - Formalin-fixed paraffin-embedded human placenta stained with hCG beta Monoclonal Antibody (SPM105)
Immunohistochemistry-Paraffin: Chorionic Gonadotropin beta Chain (HCG beta) Antibody (SPM105) [NBP2-34345] - Formalin-fixed paraffin-embedded human placenta stained with hCG beta Monoclonal Antibody (SPM105)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
SPM105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34392AF350
Alexa Fluor 405
NBP2-34392AF405
Alexa Fluor 488
NBP2-34392AF488
Alexa Fluor 532
NBP2-34392AF532
Alexa Fluor 594
NBP2-34392AF594
Alexa Fluor 647
NBP2-34392AF647
Alexa Fluor 700
NBP2-34392AF700
Alexa Fluor 750
NBP2-34392AF750
Biotin
NBP2-34392B
DyLight 350
NBP2-34392UV
DyLight 405
NBP2-34392V
DyLight 488
NBP2-34392G
DyLight 550
NBP2-34392R
DyLight 594
NBP2-34392DL594
DyLight 650
NBP2-34392C
DyLight 680
NBP2-34392FR
DyLight 755
NBP2-34392IR
FITC
NBP2-34392F
HRP
NBP2-34392H
Janelia Fluor 549
NBP2-34392JF549
Janelia Fluor 646
NBP2-34392JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-34392-0.1 mg
IHC-Prediluted
NBP2-45125
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Chorionic Gonadotropin beta Chain (hCG beta) Antibody (SPM105) Summary

Immunogen
Recombinant hCG beta protein
Localization
Cytoplasmic, Secreted
Marker
Pregnancy & Choriocarcinoma Marker
Specificity
This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CGB3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
ELISA (For coating, order NBP2-34392 (BSA free version of NBP2-34345)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (SPM105)

  • CGB
  • CGB3
  • CGB3choriogonadotropin subunit beta
  • CGB5
  • CGB7
  • CGB8
  • CG-Beta
  • Choriogonadotropin Subunit Beta
  • Chorionic gonadotrophin chain beta
  • chorionic gonadotropin beta 3 subunit
  • Chorionic Gonadotropin beta Chain
  • chorionic gonadotropin beta subunit
  • chorionic gonadotropin, beta polypeptide
  • hCGB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Chromogranin B Antibody [NBP1-80781] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80781] - Staining of human liver.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80781] - Staining of human adrenal gland shows high expression.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Serpin A6/Cortisol
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Western blot shows lysates of rat olfactory bulb tissue, rat liver tissue, rat brain (cerebellum) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A6 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin A6 was detected in perfusion fixed frozen sections of rat brain (preoptic nucleus) using Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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LH beta Antibody
(LHb/1214)
NBP2-45130
Immunohistochemistry-Paraffin: LH beta Antibody (LHb/1214) [NBP2-45130] - Formalin-fixed, paraffin-embedded human Pituitary stained with LH-beta Monoclonal Antibody (LHb/1214).
Immunohistochemistry-Paraffin: LH beta Antibody (LHb/1214) [NBP2-45130] - Formalin-fixed, paraffin-embedded human Pituitary stained with LH-beta Monoclonal Antibody (LHb/1214).
SDS-Page: LH beta Antibody (LHb/1214) [NBP2-45130]
Species: Hu
Applications: Flow, IHC, IHC-P, PAGE, IF
Chorionic
Gonadotropin alpha Ch ...
NBP2-67282
Immunocytochemistry/Immunofluorescence: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Staining FSH in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Analysis of paraffin-embedded mouse placenta tissue using anti-FSH antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: hCG Alpha (Intact) Antibody (AH33-72) [NBP2-67282] - Staining FSH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
PLOD1 Antibody
NBP2-38770
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis using Anti-PLOD1 antibody NBP2-38770 (A) shows similar pattern to independent antibody NBP2-31885 (B).
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
(2 Publications)
Species: Hu, Mu
Applications: WB
Chromogranin A
Antibody
NB120-15160
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.
Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(13 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
Cathepsin G
Antibody
NBP2-33498
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining in human bone marrow and cerebral cortex tissues using anti-CTSG antibody. Corresponding CTSG RNA-seq data are presented for the same tissues.
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CGB2 Antibody (4F8)
H00114336-M02
Western Blot: CGB2 Antibody (4F8) [H00114336-M02] - detection against Immunogen (31.9 KDa).
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Cytosolic
beta-Glucosidase/GBA3 ...
NBP2-00521
Western Blot: GBA3 Antibody (1F1) [NBP2-00521] Analysis of extracts (35ug) from 9 different cell lines by using anti-GBA3 monoclonal antibody.
Immunohistochemistry: Cytosolic beta-Glucosidase/GBA3 Antibody (1F1) [NBP2-00521] - GBA3 Antibody (1F1) [NBP2-00521] - Staining of paraffin-embedded Human prostate tissue using anti-GBA3 mouse monoclonal antibody.
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Species: Hu
Applications: WB, Flow, IHC, IHC-P
TRAPPC9 Antibody
NB100-55735
TRAPPC9 Antibody
(1 Publication)
Species: Hu, Mu, Bv, Ca, Ch, Pm
Applications: WB, ICC/IF, IP
Adenine Nucleotide
Translocase 1 Antibod ...
NBP1-59594
Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Sample Tissue: Human RPMI-8226 Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies of pinealocytes and their processes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP1-59594] - RPMI 8226 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu, Mu, Rt, Bv, Ca, Eq, Gt, GP, Rb, Sh, Ze
Applications: WB, ICC/IF, IHC
ACAA2 Antibody
(OTI1C10)
NBP2-46477
Western Blot: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ACAA2 Antibody (1C10) [NBP2-46477] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.
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Chromogranin C
Antibody
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Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-15895] - Paraffin-embedded mouse fore brain. Chromogranin C antibody dilution: 1:500.
Western Blot: Chromogranin C Antibody [NBP2-15895] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
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Applications: WB, IHC, IHC-P
CD5 Antibody
[Unconjugated]
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Human CD5 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon  Monoclonal Antibody (Catalog # MAB100) and Human CD28 Monoclonal Antibody (Catalog # MAB342), in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 2‑8 μg/mL.
(1 Publication)
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Applications: WB, Simple Western, Flow, AgAct, CyTOF-ready, ICC
Pea3 Antibody
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Western Blot: Pea3 Antibody (3G9-1B9) [H00002118-M01] - Analysis of ETV4 expression in transfected 293T cell line by ETV4 monoclonal antibody (M01), clone 3G9-1B9.Lane 1: ETV4 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.
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Galectin 13
Antibody (PP13/1161)
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Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Spleen stained with Galectin-13MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Placenta stained with Galectin-13 MAb (PP13/1161).
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BMP-4
[Unconjugated]
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1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation. 
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Placental
Lactogen/CSH1 Antibod ...
NBP2-46376
Western Blot: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSH1.
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Placental Lactogen/CSH1 Antibody (2G4) [NBP2-46376] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications: WB, IHC

Publications for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (NBP2-34345) (0)

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34392AF350
Alexa Fluor 405 NBP2-34392AF405
Alexa Fluor 488 NBP2-34392AF488
Alexa Fluor 532 NBP2-34392AF532
Alexa Fluor 594 NBP2-34392AF594
Alexa Fluor 647 NBP2-34392AF647
Alexa Fluor 700 NBP2-34392AF700
Alexa Fluor 750 NBP2-34392AF750
Biotin NBP2-34392B
DyLight 350 NBP2-34392UV
DyLight 405 NBP2-34392V
DyLight 488 NBP2-34392G
DyLight 550 NBP2-34392R
DyLight 594 NBP2-34392DL594
DyLight 650 NBP2-34392C
DyLight 680 NBP2-34392FR
DyLight 755 NBP2-34392IR
FITC NBP2-34392F
HRP NBP2-34392H
Janelia Fluor 549 NBP2-34392JF549
Janelia Fluor 646 NBP2-34392JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CGB3
Uniprot