Chorein Antibody

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85642] - Staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85642] - Staining of human colon shows strong cytoplasmic granular positivity in glandular cells.

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85642] - Staining of human kidney shows strong cytoplasmic granular positivity in cells in tubules.

Immunohistochemistry-Paraffin: Chorein Antibody [NBP1-85642] - Staining of human testis shows strong cytoplasmic granular positivity in cells in seminiferous ducts.

Product Discontinued

Chorein Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KCGLVMLNNLVKAFTEAATGSSADFVKDLAPFMILNSLGLTISVSPSDSFSVLNIPMAKSYVLKNGESLSMDYIRTKDNDHFNAMTSLSSKLFFILLTPVNHSTADKIPLTKVGRRLYTVRHRESGVERSIVCQIDTVEGSKKVTIRSPV
• Predicted Species: Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS13A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Chorein Protein (NBP1-85642PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Chorein Antibody

• CHAC
• chorea-acanthocytosis protein
• vacuolar protein sorting 13 homolog A (S. cerevisiae)
• vacuolar protein sorting 13A

Background

Chorein may control steps in the cycling of proteins through the trans-Golgi network to endosomes, lysosomes and the plasma membrane. Mutations in this gene cause the autosomal recessive disorder chorea-acanthocytosis. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chorein Antibody (NBP1-85642)(1)

Publication using NBP1-85642

• Morisaki T, Yashiro M, Kakehashi A, et al. Comparative Proteomics Analysis of Gastric Cancer Stem Cells PLos One. 2014-11-07 [PMID: 25379943] (IHC-P, Human)
  
  Details:
  Chorein/VPS13A antibody used for IHC- P at 1:500 dilution on gastric cancer tissue specimens - antigen retrieval for 10 minutes at 105C by autoclave in Target Retrieval Solution, endogenous peroxidase blocked with 3%H2O2, On/4C incubation for primary, streptavidin-peroxidase-DAB detection (Figure 3).

Bioinformatics

• Gene Symbol: VPS13A
• Entrez:
  • 23230(Human)
• Uniprot:
  • Q96RL7(Human)