Recombinant Human CHMP2A His Protein

SDS-Page: Recombinant Human CHMP2A Protein [NBP1-30206] - CHMP2A, 27.2 kDa (242aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human CHMP2A His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-222 of Human CHMP2A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CHMP2A
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl 1 mM DTT, and 30% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human CHMP2A His Protein

• BC-2
• BC2VPS2 homolog A
• CHMP2a
• CHMP2hVps2-1
• chromatin modifying protein 2A
• Chromatin-modifying protein 2a
• Putative breast adenocarcinoma marker BC-2
• Vacuolar protein sorting-associated protein 2-1
• vps2-1
• VPS2Aputative breast adenocarcinoma marker (32kD)
• VPS2charged multivesicular body protein 2a

Background

CHMP2A, also known as chromatin modifying protein 2A, belongs to the SNF7 family and it is component of the ESCRT III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. Recombinant CHMP2A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CHMP2A