CHD3 Antibody (2G4)

Images

 
Western Blot: CHD3 Antibody (2G4) [NBP1-51593] - Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (2G4) [NBP1-51593] - Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CHD3 Antibody (2G4) [NBP1-51593] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
Flow Cytometry: CHD3 Antibody (2G4) [NBP1-51593] - Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHD3 Antibody (2G4) Summary

Immunogen
Purified recombinant fragment of human CHD3 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHD3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
226 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-51593.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for CHD3 Antibody (2G4)

  • ATP-dependent helicase CHD3
  • CHD-3
  • chromodomain helicase DNA binding protein 3
  • chromodomain-helicase-DNA-binding protein 3
  • EC 3.6.1
  • EC 3.6.4.12
  • hZFH
  • Mi-2 autoantigen 240 kDa protein
  • Mi-2a
  • Mi2-ALPHA
  • ZFH
  • Zinc finger helicase
  • zinc-finger helicase (Snf2-like)

Background

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHD3 Antibody (NBP1-51593)(1)

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHD3 Antibody (NBP1-51593). (Showing 1 - 1 of 1 FAQ).

  1. Do you know what part of the protein CHD3 Antibody (2G4) (NBP1-51593) was raised against?
    • The AA range is: 193-479.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHD3