Western Blot: CHD3 Antibody (2G4) [NBP1-51593] - Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (2G4) [NBP1-51593] - Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CHD3 Antibody (2G4) [NBP1-51593] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
Flow Cytometry: CHD3 Antibody (2G4) [NBP1-51593] - Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).
Hu, MuSpecies Glossary
WB, ELISA, Flow, ICC/IF, IHC, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for CHD3 Antibody (2G4)
ATP-dependent helicase CHD3
chromodomain helicase DNA binding protein 3
chromodomain-helicase-DNA-binding protein 3
Mi-2 autoantigen 240 kDa protein
Zinc finger helicase
zinc-finger helicase (Snf2-like)
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.