CGRP2 Antibody (1057815) [Unconjugated]

Recombinant Human CGRP2 protein was serially diluted 2-fold and captured by Mouse Anti-Human CGRP2 Monoclonal Antibody (MAB11382) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB11323) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Procalcitonin was detected in immersion fixed TT human medullary thyroid cancer cell line (positive) and absent in THP‑1 human acute monocytic leukemia cell line (negative) using Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB11323) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Procalcitonin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB11323) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: ELISA, IHC, ICC/IF
• Clone: 1057815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CGRP2 Antibody (1057815) [Unconjugated] Summary

• Immunogen: Human CGRP synthetic peptide (Tocris Catalog # 3012)
  Accession # P10092
• Specificity: Detects Human Procalcitonin in direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CGRP2 Antibody (1057815) [Unconjugated]

• beta-CGRP
• CALC2
• Calcitonin 2
• CGRP2
• CGRP-II
• FLJ30166

Background

Calcitonin gene-related peptide or CGRP is a 37-amino acid neuropeptide formed by alternative splicing of the calcitonin/CGRP gene. CGRP is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons and induces vasodilation of a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P10092()