CEP350 Antibody (CL3423) - Azide and BSA Free

Staining of A431 cells using the anti-CEP350 monoclonal antibody) .

Staining of human breast cancer shows centrosome-like immunoreactivity in tumor cells.

Staining of human colon shows centrosome-like positivity in lymphoid cells in lamina propria.

Staining of human placenta shows centrosome-like immunoreactivity in trophoblast cells.

Staining of U2OS cells using the anti-CEP350 monoclonal antibody) .

Staining of U251 cells using the anti-CEP350 monoclonal antibody) .

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL3423
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CEP350 Antibody (CL3423) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CEP350 Antibody (CL3423) - Azide and BSA Free (NBP2-59028) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q5VT06, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEP350
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CEP350 Antibody (CL3423) - Azide and BSA Free

• CAP350GM133
• centrosomal protein 350kDa
• centrosome-associated protein 350
• Centrosome-associated protein of 350 kDa
• Cep350
• FLJ38282
• FLJ44058
• KIAA0480centrosome associated protein 350

Background

CAP350 (Centrosome-Associated Protein 350) has been shown to localize to the centrosome as well as to dots in the pericentrosomal area. CAP350 functions to stabilize and anchor microtubules to the centrosome and participates in the maintenance of a pericentrosomal Golgi ribbon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEP350