Centrin 1 Antibody (CETN1/9877) - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CETN1/9877
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Centrin 1 Antibody (CETN1/9877) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-44585).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant fragment (around aa1-172) of human Centrin 1 protein (Uniprot: Q12798). Exact sequence is proprietary.
• Localization: Cell projection, Centrosome, Cytoplasm, Cytoskeleton
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: CETN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry : 1-2ug/ml
  • Western Blot : 2-4ug/ml
• Application Notes: Immunohistochemistry: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Centrin 1 Antibody (CETN1/9877) - Azide and BSA Free

• calcium binding protein
• Caltractin isoform 2
• caltractin
• CEN1centrin-1
• centrin, EF-hand protein, 1
• CETN
• EF-hand protein

Background

Centrin 1 is encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CETN1