Centaurin beta 2 Antibody (S05-8H3)

Western Blot: Centaurin beta 2 Antibody (S05-8H3) [NBP3-15011] - Western blot detection of Centaurin beta 2 in Hela, CHO-K1, C6, Jurkat cell lysates using Centaurin beta 2 Rabbit mAb (1:500 diluted). Predicted band size: 88kDa. Observed band size: 88kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-8H3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Centaurin beta 2 Antibody (S05-8H3) Summary

• Description: Novus Biologicals Rabbit Centaurin beta 2 Antibody (S05-8H3) (NBP3-15011) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Centaurin beta 2 (Uniprot#: Q15057)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACAP2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/2000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Centaurin beta 2 Antibody (S05-8H3)

• Arf GAP with coiled coil, ANK repeat and PH domains 2
• ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
• centaurin, beta 2
• Centaurin-beta-2
• CENTB2centaurin-beta-2
• CNT-B2
• KIAA0041arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Background

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACAP2