Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, IHC
Host: Rabbit Polyclonal
Species: Hu, Mu, Rt, Po, Ca
Applications: WB, ELISA, IHC
Host: Goat Polyclonal
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC
Host: Rabbit Polyclonal
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ELISA, MA, AP
Species: Hu
Applications: AC
Species: Hu
Applications: AC
Description
Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.
Bioinformatics
Entrez |
Human Mouse |
Uniprot |
Human Human Human |
Product By Gene ID |
23527 |
Alternate Names |
- Arf GAP with coiled coil, ANK repeat and PH domains 2
- ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
- centaurin, beta 2
- Centaurin-beta-2
- CENTB2centaurin-beta-2
- CNT-B2
- Cnt-b2
- KIAA0041arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
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