CELSR2 Antibody

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human CELSR2. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human CELSR2 Antigen Affinity-purified ...read more
CELSR2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739) at 10 µg/mL overnight at 4 °C. Tissue was ...read more
SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Human CELSR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6739, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CELSR2 Antibody Summary

Immunogen
E. coli-derived recombinant human CELSR2
Cys51-Phe231
Accession # Q9HCU4
Specificity
Detects human CELSR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CELSR2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF6739 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CELSR2 Antibody

  • cadherin EGF LAG seven-pass G-type receptor 2
  • Cadherin family member 10
  • cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
  • CDHF10
  • CDHF10FLJ34118
  • CELSR2
  • EGFL2
  • EGFL2FLJ45143
  • EGF-like protein 2
  • EGF-like-domain, multiple 2
  • epidermal growth factor-like 2
  • Epidermal growth factor-like protein 2
  • Flamingo homolog 3
  • Flamingo1
  • FLJ45845
  • KIAA0279FLJ42737
  • MEGF3
  • MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog
  • Multiple EGF-like domains protein 3
  • multiple epidermal growth factor-like domains 3
  • Multiple epidermal growth factor-like domains protein 3

Background

CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CELSR2 Antibody (AF6739)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CELSR2
Uniprot