Cdk6 Antibody (SD20-50)

Western Blot: Cdk6 Antibody (SD20-50) [NBP2-67758] - Western blot analysis of Cdk6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (SD20-50) [NBP2-67758] - Staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cdk6 Antibody (SD20-50) [NBP2-67758] - Analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.

Flow Cytometry: Cdk6 Antibody (SD20-50) [NBP2-67758] - Analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (SD20-50) [NBP2-67758] - Staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (SD20-50) [NBP2-67758] - Staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cdk6 Antibody (SD20-50) [NBP2-67758] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.

Product Discontinued

Cdk6 Antibody (SD20-50) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cdk6 aa 277-326 / 326. (SwissProt: Q00534 Human)
• Localization: Cytoplasm, Nucleus, Cell projection.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdk6 Antibody (SD20-50)

• Cdk6
• CDKN6
• Cell division protein kinase 6
• cyclin-dependent kinase 6
• EC 2.7.11
• EC 2.7.11.22
• PLSTIREMGC59692
• Serine/threonine-protein kinase PLSTIRE

Background

Cdk6 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK6