Cdk6 Antibody (DCS-83.1) - BSA Free

Western Blot: Cdk6 Antibody (DCS-83.1) [NB100-65577] - Analysis of Molt-4 acute lymphoblastic derived T-cell lysate probed with Mouse anti Human Cdk6 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence.

Product Discontinued

Cdk6 Antibody (DCS-83.1) - BSA Free Summary

• Immunogen: Recombinant cdk6 protein
• Specificity: Recognizes human cyclin dependent kinase 6 (Cdk6), a 40kD protein which plays an important role in the progression of G1 to S.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Control:

  Cdk6 Overexpression Lysate

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cdk6 Antibody (DCS-83.1) - BSA Free

• Cdk6
• CDKN6
• Cell division protein kinase 6
• cyclin-dependent kinase 6
• EC 2.7.11
• EC 2.7.11.22
• PLSTIREMGC59692
• Serine/threonine-protein kinase PLSTIRE

Background

Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK6
• Entrez:
  • 1021(Human)
• Uniprot:
  • Q00534(Human)