CDC37 [p Ser13] Antibody (SD08-50)

Western Blot: CDC37 [p Ser13] Antibody (SD08-50) [NBP2-67807] - Analysis of Phospho-CDC37(S13) on different lysates using anti-Phospho-CDC37(S13) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Jurkat

Western Blot: CDC37 [p Ser13] Antibody (SD08-50) [NBP2-67807] - Western blot analysis of CDC37 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Product Discontinued

CDC37 [p Ser13] Antibody (SD08-50) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37. (SwissProt: Q16543 Human; SwissProt: Q61081 Mouse; SwissProt: Q63692 Rat)
• Modification: p Ser13
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC37
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC37 [p Ser13] Antibody (SD08-50)

• CDC37 (cell division cycle 37, S. cerevisiae, homolog)
• CDC37 cell division cycle 37 homolog (S. cerevisiae)
• CDC37 cell division cycle 37 homolog
• CDC37A
• cell division cycle 37 homolog (S. cerevisiae)
• Hsp90 chaperone protein kinase-targeting subunit
• hsp90 co-chaperone Cdc37
• P50CDC37

Background

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC37