CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2

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Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3734G, filled histogram) or isotype control antibody (Catalog # IC005G, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
587702
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 488

CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2 Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
Gln24-His384
Accession # AAH06676
Specificity
In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
CD97
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
    12 months from date of receipt, 2 to 8 degreesC as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2

  • CD97

Background

CD97 is an approximately 90-100 kDa, 818 amino acid (aa) N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. Mouse CD97 contains a 510 aa N-terminal extracellular region with four EGF-like domains and a short 45 aa cytoplasmic tail. It undergoes proteolytic processing between Thr513-Ser514 to generate a 68-80 kDa N-terminal alpha -chain, and a 26-28 kDa membrane-bound beta -chain. The two chains remain non-covalently linked on the cell membrane with dissociation and solubilization of the alpha -chain under select conditions. CD97 in mouse has an alternately spliced variant 2 (V2) that exhibits a 94 aa deletion, resulting in the loss of the third EGF-like repeat (aa 69-119). CD97 is expressed on monocytes, macrophages, neutrophils, T cells, B cell subsets, dendritic cells, mesenchymal stem cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, CD90, and alpha V beta 3 Integrin plus alpha 5 beta 1 Integrin. Interaction with these molecules may vary between splice forms of CD97. On the cell surface, the alpha beta heterodimer may "homodimerize", and form a true heterodimer with the 7-transmembrane LPAR1. CD97 participates in neutrophil migration, host defense, and angiogenesis. It also facilitates chemokine binding to PMNs, and inhibits select cell migration by downregulating MMP activity. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD97
Entrez
Uniprot