CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2

Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3734G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 587702
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2 Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2
  Gln24-His384
  Accession # AAH06676
• Specificity: In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD97
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD97 Isoform 1 Antibody (587702) [Alexa Fluor® 488] - v2

• CD97
• TM7LN1

Background

CD97 is an approximately 90-100 kDa, 818 amino acid (aa) N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. Mouse CD97 contains a 510 aa N-terminal extracellular region with four EGF-like domains and a short 45 aa cytoplasmic tail. It undergoes proteolytic processing between Thr513-Ser514 to generate a 68-80 kDa N-terminal alpha -chain, and a 26-28 kDa membrane-bound beta -chain. The two chains remain non-covalently linked on the cell membrane with dissociation and solubilization of the alpha -chain under select conditions. CD97 in mouse has an alternately spliced variant 2 (V2) that exhibits a 94 aa deletion, resulting in the loss of the third EGF-like repeat (aa 69-119). CD97 is expressed on monocytes, macrophages, neutrophils, T cells, B cell subsets, dendritic cells, mesenchymal stem cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, CD90, and  alpha V beta 3 Integrin plus alpha 5 beta 1 Integrin. Interaction with these molecules may vary between splice forms of CD97. On the cell surface, the alpha beta  heterodimer may "homodimerize", and form a true heterodimer with the 7-transmembrane LPAR1. CD97 participates in neutrophil migration, host defense, and angiogenesis. It also facilitates chemokine binding to PMNs, and inhibits select cell migration by downregulating MMP activity. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD97
• Uniprot:
  • AAH06676()