CD2BP2 Antibody

Western Blot: CD2BP2 Antibody [NB100-1526] - (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CD2BP2 Antibody Summary

• Immunogen: Peptide with sequence PKRKVTFQGVGDE-C corresponding to N-Terminus according to NP_006101.1.
• Epitope: N Terminus
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD2BP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 10 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human lymph node, thymus and tonsil lysates (calculated MW of 37.6 kDa band according to NP_006101.1). The observed molecular weights correspond to earlier findings is reported in scientific literature with different antibodies (Nishizawa et al, Proc Natl Acad Sci U S A. 1998 Dec 8;95(25): 14897-902). IHC-P: Human spleen shows nucleolar staining in select splenocytes.
• Control:

  Human Thymus Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD2BP2 Antibody

• CD2 (cytoplasmic tail) binding protein 2
• CD2 antigen (cytoplasmic tail) binding protein 2
• CD2 antigen (cytoplasmic tail)-binding protein 2
• CD2 antigen cytoplasmic tail-binding protein 2
• CD2 binding protein 2
• CD2 cytoplasmic domain binding protein 2
• CD2 cytoplasmic domain-binding protein 2
• CD2 tail-binding protein 2
• FWP010
• KIAA1178
• LIN1
• Snu40
• U5 snRNP 52K protein
• U5-52K

Background

CD2BP2 is an intracellular protein which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17 amino acid motif also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD2BP2 Antibody (NB100-1526)(1)

Publication using NB100-1526

• Nishizawa K, Freund C, Li J, Wagner G, Reinherz EL. Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation . Proc Natl Acad Sci U S A;95(25):14897-902. 1998-12-08 [PMID: 9843987]

Bioinformatics

• Gene Symbol: CD2BP2
• Entrez:
  • 10421(Human)
  • 70233(Mouse)
• Uniprot:
  • NP_006101.1(Human)