Recombinant Human Caspase-6 Protein

Recombinant Human Caspase-6 Protein [NBP1-99589]

Product Discontinued

Recombinant Human Caspase-6 Protein Summary

• Description: Recombinant active human caspase-6 expressed in E. coli and fused with His tag (Uniprot# P55212-1 (isoform alpha)).
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab52157)
• Details of Functionality: Specific Activity: 13,000 units/mg. One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP6
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • In vitro assay
  • SDS-Page
• Application Notes: Use in In vitro reported in scientific literature (PMID:34862482).Molecular Weight: large (18 ) and small (11).
• Theoretical MW: 14.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -70C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per ul in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-6 Protein

• Apoptotic protease Mch-2
• CASP6
• CASP-6
• caspase 6, apoptosis-related cysteine peptidase
• caspase 6, apoptosis-related cysteine protease
• Caspase6
• Caspase-6
• EC 3.4.22
• Mch2
• MCH2EC 3.4.22.59

Background

Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Caspase-6 Recombinant Protein (NBP1-99589)(1)

Publication using NBP1-99589

• Valionyte E, Yang Y, Griffiths SA et al. The caspase-6-p62 axis modulates p62 droplets based autophagy in a dominant-negative manner Cell death and differentiation 2021-12-03 [PMID: 34862482] (In vitro)

Bioinformatics

• Gene Symbol: CASP6