Caspase-6 Assay Kit (Colorimetric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Cell and tissue lysates

Order Details

Caspase-6 Assay Kit (Colorimetric) Summary

Description
Caspase-6 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-6 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.

  • Detection method- Fluorescence (Ex/Em 400/505 nm)
  • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit Type
Assay Kit (Colorimetric)
Gene
CASP6

Applications/Dilutions

Application Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. VEID-pNA (4 mM)
  2. 2X Reaction Buffer
  3. DTT (1 M)
  4. Dilution Buffer
  5. Cell Lysis Buffer

Alternate Names for Caspase-6 Assay Kit (Colorimetric)

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CASP6
Entrez