Human, Mouse, Rat Caspase-6 ELISA Kit (Colorimetric)

Product Discontinued

Human, Mouse, Rat Caspase-6 ELISA Kit (Colorimetric) Summary

• Description: CASP6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CASP6 expression in cultured cells. For storage and other information please see the protocol under the Product Pdf's section on the product page of our website.
• Standard Curve Range: > 5000 Cells
• Sensitivity: n/a
• Assay Type: Indirect ELISA kit (Cell-Based)
• Sample Volume: n/a
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CASP6

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Useful for qualification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse, Rat Caspase-6 ELISA Kit (Colorimetric)

• Apoptotic protease Mch-2
• CASP6
• CASP-6
• caspase 6, apoptosis-related cysteine peptidase
• caspase 6, apoptosis-related cysteine protease
• Caspase6
• Caspase-6
• EC 3.4.22
• Mch2
• MCH2EC 3.4.22.59

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Caspase-6 ELISA Kit (KA2638). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CASP6