Caspase-6 Antibody (6CSP01 (7.1.65))

Images

 
Western Blot: Caspase-6 Antibody (6CSP01 (7.1.65)) [NB600-1000] - IP followed by Western Blot analysis of Caspase- 6 on Human Cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
6CSP01 (7.1.65)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Caspase-6 Antibody (6CSP01 (7.1.65)) Summary

Immunogen
Recombinant human Caspase 6 prodomain.
Epitope
Caspase 6 prodomain.
Localization
Cytoplasmic.
Specificity
Caspase 6 (6CSP01 (7.1.65))
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunoprecipitation 2ug/mg
Application Notes
Immunoprecipitation (denatured verified, with protein G): use at a concentration of 2ug/mg of lysate. Not tested in any other applications. Optimal dilutions/concentrations should be determined by the end user.
Control
Caspase-6 Lysate (NBL1-08715)
Caspase-6 Lysate (NBP2-65003)

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Caspase-6 Antibody (6CSP01 (7.1.65))

  • Apoptotic protease Mch-2
  • CASP6
  • CASP-6
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6, apoptosis-related cysteine protease
  • Caspase6
  • Caspase-6
  • EC 3.4.22
  • Mch2
  • MCH2EC 3.4.22.59

Background

Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP6
Entrez