CARS Antibody

Western Blot: CARS Antibody [H00000833-B01P] - Analysis of CARS expression in human liver.

Immunocytochemistry/Immunofluorescence: CARS Antibody [H00000833-B01P] - Analysis of purified antibody to CARS on HeLa cell. (antibody concentration 7 ug/ml)

Western Blot: CARS Antibody [H00000833-B01P] - Analysis of CARS expression in HeLa.

Western Blot: CARS Antibody [H00000833-B01P] - Analysis of CARS expression in transfected 293T cell line by CARS polyclonal antibody. Lane 1: CARS transfected lysate(82.28 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

CARS Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: CARS (NP_001742.1, 1 a.a. - 748 a.a.) full-length human protein. MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQNGSFQ
• Specificity: CARS - cysteinyl-tRNA synthetase,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: CARS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CARS Antibody

• CARS1
• CysRS
• cysteine translase
• cysteine tRNA ligase 1, cytoplasmic
• Cysteine--tRNA ligase
• cysteinyl-tRNA synthetase
• cysteinyl-tRNA synthetase, cytoplasmic
• EC 6.1.1
• EC 6.1.1.16
• MGC:11246

Background

This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CARS Antibody (H00000833-B01P)(1)

Publication using H00000833-B01P

• Kuo ME, Theil AF, Kievit A et al. Cysteinyl-tRNA Synthetase Mutations Cause a Multi-System, Recessive Disease That Includes Microcephaly, Developmental Delay, and Brittle Hair and Nails Am. J. Hum. Genet. 2019-02-26 [PMID: 30824121] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CARS1
• Entrez:
  • 833(Human)
• Uniprot:
  • NP_001742.1(Human)