Carbonic Anhydrase VB/CA5B 293T Cell Transient Overexpression Lysate

CA5B transfected lysate.

Lane 1: CA5B transfected lysate (34.98 KDa) Lane 2: Non-transfected lysate.

Product Discontinued

Carbonic Anhydrase VB/CA5B 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-CA5B antibody by Western Blots. Plasmid: pCMV-CA5B full-length
• Specificity: Carbonic Anhydrase VB/CA5B 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: CA5B

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carbonic Anhydrase VB/CA5B 293T Cell Transient Overexpression Lysate

• CA5B
• Carbonate dehydratase VB
• carbonic anhydrase 5B, mitochondrial
• Carbonic Anhydrase VB
• carbonic anhydrase VB, mitochondrial
• carbonic dehydratase
• CAVB
• CA-VB
• EC 4.2.1.1
• MGC39962

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CA5B