Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

ELISA: Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric) [NBP2-70059] - Samples were spiked with high concentrations of Human Carbonic Anhydrase VB/CA5B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric) [NBP2-70059] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CA5B in samples. No significant cross-reactivity or interference between Human CA5B and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.84%
• Intra-Assay: CV% < 5.28%
• Spike Recovery: 86-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CA5B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Carbonic Anhydrase VB/CA5B ELISA Kit (Colorimetric)

• CA5B
• Carbonate dehydratase VB
• carbonic anhydrase 5B, mitochondrial
• Carbonic Anhydrase VB
• carbonic anhydrase VB, mitochondrial
• carbonic dehydratase
• CAVB
• CA-VB
• EC 4.2.1.1
• MGC39962

Background

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide.CAs show extensive diversity in their distribution and subcellular localization.They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA VB, also known as carbonate dehydratase VB, is one of two isoforms of CA V. It localizes to the mitochondria and is involved in metabolic processes. CA VB is predominantly expressed in heart, pancreas, lung, placenta, kidney and skeletal muscle. It exhibits highest homology with family member CA VA (the second isoform of CA V); however, unlike CA VA, it is not expressed in the liver, suggesting that it plays a significantly different physiological role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CA5B