Carbohydrate Sulfotransferase 2/CHST2 Antibody

Images

 
Western Blot: Carbohydrate sulfotransferase 2 Antibody [NBP2-15696] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Carbohydrate sulfotransferase 2 Antibody [NBP2-15696] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Carbohydrate Sulfotransferase 2/CHST2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CHST2. The exact sequence is proprietary.
Localization
Golgi apparatus; trans-Golgi network membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHST2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Carbohydrate Sulfotransferase 2/CHST2 Antibody

  • C6ST
  • carbohydrate (chondroitin 6/keratan) sulfotransferase 2
  • carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
  • Carbohydrate Sulfotransferase 2
  • CHST2
  • EC 2.8.2.-
  • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
  • GlcNAc6ST-1
  • GN6ST
  • Gn6ST-1
  • GST2
  • GST-2
  • N-acetylglucosamine 6-O-sulfotransferase 1

Background

N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CHST2
Entrez
Uniprot