Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

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Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody [Biotin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 1/CHST1
Arg24-Ser411
Accession # O43916
Specificity
Detects human Carbohydrate Sulfotransferase 1/CHST1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse CHST1 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST2, rhCHST5, and rmCHST7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CHST1
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 1/CHST1/KS6ST Antibody [Biotin]

  • 6-O-sulfotransferase 1
  • C6ST
  • carbohydrate (chondroitin 6/keratan) sulfotransferase 1
  • carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
  • Carbohydrate Sulfotransferase 1
  • CHST1
  • EC 2.8.2
  • Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
  • GST-1
  • Keratan sulfate Gal-6 sulfotransferase
  • KS6ST
  • KSGal6ST
  • KSGal6STEC 2.8.2.21
  • KSST

Background

The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal-6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse CHST1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF5316

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Bioinformatics

Gene Symbol CHST1
Uniprot