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Human CaMKIIN2 ELISA Kit (Colorimetric)

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Human CaMKIIN2 ELISA Kit (Chemiluminescence) - Standard Curve Reference
Human CaMKIIN2 ELISA Kit (Chemiluminescence) - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

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Human CaMKIIN2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CAMK2N2 in samples. No significant cross-reactivity or interference between Human CAMK2N2 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.97%
Intra-Assay
CV% < 5.97%
Spike Recovery
86-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CAMK2N2

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Wash Buffer (25×)
  3. Concentrated HRP Conjugate (100×)
  4. Certificate of Analysis
  5. Concentrated Biotinylated Detection Ab (100×)
  6. Product Description
  7. Plate Sealer
  8. Reference Standard
  9. Reference Standard & Sample Diluent
  10. HRP Conjugate Diluent
  11. Micro ELISA Plate(Dismountable)
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human CaMKIIN2 ELISA Kit (Colorimetric)

  • calcium/calmodulin-dependent protein kinase II inhibitor 2
  • CaM-KII inhibitory protein
  • CAMKIIN
  • CaM-KIIN

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
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Calmodulin
Antibody (2D1)
NB120-2860
Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
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Calmodulin 3
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NBP2-15669
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. Western Blot shows that hsa-miR-4709-3p inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection;4709: hsa-miR-4709-3p transfection. Image collected and cropped by CiteAb from the following publication (https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-019-1512-x) licensed under a CC-BY licence.
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
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NBP2-19807
Western Blot: Phospholamban Antibody [NBP2-19807] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP2-19807] - Human colon carcinoma, using phospholamban antibody at 1:250 dilution.Antigen Retrieval: Trilogy&amp;#8482; (EDTA based, pH 8.0) buffer, 15min.
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NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NT-3 [Unconjugated]
267-N3/CF
1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human NT-3 (Catalog #267‑N3/CF) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.
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CaM Kinase I
Antibody
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Western blot shows lysates of IMR‑32 human neuroblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2&amp;nbsp;µg/mL of Sheep Anti-Human CaM&amp;nbsp;Kinase&amp;nbsp;I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7899) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog&amp;nbsp;# HAF016). A specific band was detected for CaM&amp;nbsp;Kinase&amp;nbsp;I at approximately 37&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CaM&amp;nbsp;Kinase&amp;nbsp;I was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Sheep Anti-Human CaM&amp;nbsp;Kinase&amp;nbsp;I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7899) at 1.7&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Sheep HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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ERK2 Antibody
(191801) [Unconjugate
MAB1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF007). A specific band was detected for ERK2 at approximately&amp;nbsp;44&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Antibody - Pan Specif
AF887
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Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody [NBP1-31209] - Synuclein-gamma Antibody [NBP1-31209] - HeLa cells, using gamma Synuclein antibody at 1:200 dilution.
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Immunohistochemistry-Paraffin: Secretin R Antibody [NBP1-86125] - Staining of human pancreas shows strong membranous positivity in interlobular ducts.
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IP3R1 Antibody
NBP1-21398
Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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Immunocytochemistry/Immunofluorescence: RPS12 Antibody [NBP2-94504] - Analysis of MCF-7 cells using RPS12. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPS12 Antibody [NBP2-94504] - Paraffin-embedded rat brain using RPS12.
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Immunocytochemistry/Immunofluorescence: MITF Antibody (21D1418) [NB100-56561] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MITF Antibody (21D1418) NB100-56561 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: MITF Antibody (21D1418) [NB100-56561] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of MITF (21D1418) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. Some nuclear reactivity is observed as well.
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Immunocytochemistry/Immunofluorescence: PSMD7 Antibody [NBP2-38780] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
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Bioinformatics

Gene Symbol CAMK2N2