Calpastatin Antibody (1F7E3D10)

Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.

Product Discontinued

Calpastatin Antibody (1F7E3D10) Summary

• Immunogen: Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin.
• Epitope: This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.
• Localization: Cytoplasmic and Nuclear
• Specificity: This does not cross-react with calpains or calmodulin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAST
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:5000
• Application Notes: WB: Detects an approx. 150, 125, 90 and 70 kDa proteins (predicted molecular weight: 85 kDa) from bovine skeletal muscle extract, which have been suggested to be proteins translated from different start sites of the calpastatin gene. ICC: Staining of calpastatin in porcine LLC-PK1 cells with ab5582 results in diffuse cytoplasmic staining.
• Control:

  Calpastatin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calpastatin Antibody (1F7E3D10)

• BS-17
• Calpain inhibitor
• calpastatin
• MGC9402
• Sperm BS-17 component

Background

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calpastatin Antibody (NB120-5582)(1)

Publication using NB120-5582

• Kaur J, Mencl S, Sahaboglu A et al. Calpain and PARP activation during photoreceptor cell death in P23H and S334ter rhodopsin mutant rats. PLoS One 2011-01-01 [PMID: 21765948]

Bioinformatics

• Gene Symbol: CAST
• Entrez:
  • 831(Human)
  • 25403(Rat)
• Uniprot:
  • P20811(Bovine)
  • P20810(Human)