Calpastatin Antibody (1F7E3D10)

Images

 
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
1F7E3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Calpastatin Antibody (1F7E3D10) Summary

Immunogen
Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin.
Epitope
This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.
Localization
Cytoplasmic and Nuclear
Specificity
This does not cross-react with calpains or calmodulin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAST
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
WB: Detects an approx. 150, 125, 90 and 70 kDa proteins (predicted molecular weight: 85 kDa) from bovine skeletal muscle extract, which have been suggested to be proteins translated from different start sites of the calpastatin gene. ICC: Staining of calpastatin in porcine LLC-PK1 cells with ab5582 results in diffuse cytoplasmic staining.
Control
Calpastatin Overexpression Lysate
Calpastatin Knockout HeLa Cell Lysate
Publications
Read Publication using NB120-5582.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Calpastatin Antibody (1F7E3D10)

  • BS-17
  • Calpain inhibitor
  • calpastatin
  • MGC9402
  • Sperm BS-17 component

Background

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calpastatin Antibody (NB120-5582)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

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