CACNB1 Antibody (S7-18)

Western Blot: CACNB1 Antibody (S7-18) [NBP2-12907] - analysis of Rat brain membrane lysate showing detection of CACNB1 Calcium Channel protein using Mouse Anti-CACNB1 Calcium Channel Monoclonal Antibody, Clone S7-18 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-CACNB1 Calcium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: CACNB1 Antibody (S7-18) [NBP2-12907]

Product Discontinued

CACNB1 Antibody (S7-18) Summary

• Immunogen: Synthetic peptide amino acids 19-34 of rat CavB1
• Localization: Cell Membrane
• Specificity: Detects approx 80, 55kDa. No cross-reactivity against Cav Beta4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CACNB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Cav beta 1 Antibody was sufficient for detection of CavB1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CACNB1 Antibody (S7-18)

• CAB1
• CACNB1
• CACNLB1
• CACNLB1calcium channel, L type, beta 1 polypeptide
• Calcium channel voltage-dependent subunit beta 1
• calcium channel, voltage-dependent, beta 1 subunit
• CCHLB1
• dihydropyridine-sensitive L-type, calcium channel beta-1 subunit
• MGC41896
• voltage-dependent L-type calcium channel subunit beta-1

Background

1 ug/mL was sufficient for detection of Cav beta1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNB1