C5orf30 Overexpression Lysate

Images

 
Western Blot: C5orf30 Overexpression Lysate (Adult Normal) [NBL1-08490] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C5orf30.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

C5orf30 Overexpression Lysate Summary

Description
Transient overexpression lysate of chromosome 5 open reading frame 30 (C5orf30) The lysate was created in HEK293T cells, using Plasmid ID RC203510 and based on accession number NM_033211. The protein contains a C-MYC/DDK Tag.
Gene
C5ORF30

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
22.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for C5orf30 Overexpression Lysate

  • chromosome 5 open reading frame 30
  • FLJ25291
  • hypothetical protein LOC90355

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB3428
Species: Hu
Applications: WB, IHC
NBP2-14719
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-67868
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-45312
Species: Hu, Pm, Mu(-)
Applications: WB, Flow, IHC, IHC-P, IF
NBP2-14093
Species: Hu
Applications: WB, IHC, IHC-P
H00000640-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, S-ELISA
H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
NBP3-03661
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-32005
Species: Hu
Applications: WB, IHC, IHC-P
MAB342
Species: Hu
Applications: WB, AgAct
NBP2-26116
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-77533
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-87714
Species: Hu
Applications: IHC, IHC-P
NBL1-08490
Species: Hu
Applications: WB

Publications for C5orf30 Lysate (NBL1-08490) (0)

There are no publications for C5orf30 Lysate (NBL1-08490).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for C5orf30 Lysate (NBL1-08490) (0)

There are no reviews for C5orf30 Lysate (NBL1-08490). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for C5orf30 Lysate (NBL1-08490) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-08490

Additional C5orf30 Products

Bioinformatics Tool for C5orf30 Lysate (NBL1-08490)

Discover related pathways, diseases and genes to C5orf30 Lysate (NBL1-08490). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for C5orf30 Lysate (NBL1-08490)

Discover more about diseases related to C5orf30 Lysate (NBL1-08490).

Blogs on C5orf30

There are no specific blogs for C5orf30, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our C5orf30 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol C5ORF30