Biliverdin Reductase A/BLVRA Antibody

Western Blot: Biliverdin Reductase A/BLVRA Antibody [NB120-3466] - Analysis of BLVRA on HeLa cell extract.

Product Discontinued

Biliverdin Reductase A/BLVRA Antibody Summary

• Immunogen: Purified human biliverdin-IX alpha-reductase expressed in E. coli.
• Specificity: NB120-3466 detects biliverdin-IX alpha reductase (BLVRA) from human cells.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: BLVRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/ml
• Application Notes: WB: Detects an approx. 40 kDa protein representing BLVRA from HeLa cell extract.
• Control:

  Biliverdin Reductase A/BLVRA Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Biliverdin Reductase A/BLVRA Antibody

• Biliverdin Reductase A
• Biliverdin-IX alpha-reductase
• BLVR
• BLVRA
• BVR A
• BVR
• BVRA
• EC 1.3.1.24

Background

Biliverdin Reductase (BLVR) is a ubiquitously expressed, small cytoplasmic oxidoreductase that catalyzes the reduction of biliverdin to bilirubin. The first step of this process is catalyzed by heme oxygenase, an enzyme that converts heme to iron, carbon monoxide, and biliverdin. BLVR is unique among all enzymes to date in being dual pH/dual cofactor-dependent, as it requires NADH (pH 6.7) or NADPH (pH 8.7) as an electron donor. There are two isoforms of BLVR, Biliverdin-IX alpha-reductase (BLVRA), the major component of human adult liver, and biliverdin IX beta-reductase (BLVRB), found predominantly in fetal liver. The product of BLVR, bilirubin, is an important biological antioxidant, however it is also a neurotoxin and the cause of kernicterus. In addition to being an antioxidant, it inhibits kinase activity and the activity of enzymes such as protein kinase C and NADPH oxidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLVRA
• Entrez:
  • 644(Human)