beta-Arrestin 2 Antibody

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Western Blot: beta-Arrestin 2 Antibody [NB300-587] - Analysis of rat retina.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

beta-Arrestin 2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2.
Specificity
Detects recombinant rat beta-arrestin and beta-arrestin 2. This does not detect visual or cone arrestin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARRB2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 49 kDa and ~47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples.
Control Peptide
beta-Arrestin 2 Protein (NB300-587PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19776273). Rat reactivity reported in scientific literature (PMID: 19200235).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-Arrestin 2 Antibody

  • ARB2
  • ARR2
  • ARRB2
  • arrestin 3
  • Arrestin beta-2
  • arrestin, beta 2
  • BARR2
  • betaArrestin 2
  • beta-Arrestin 2
  • beta-arrestin-2
  • DKFZp686L0365

Background

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ARRB2
Entrez
Uniprot