beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta -1,4‑Galactosyltransferase 1/B4GalT1 long isoform
  Gly44-Ser398
  Accession # P15291
• Specificity: Detects human beta -1,4‑Galactosyltransferase 1/B4GalT1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: B4GALT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody

• 1,4- galactosyltransferase, polypeptide 1
• B4GalT1
• B4GAL-T1
• beta-1,4-galactosyltransferase 1
• Beta-1,4-GalTase 1
• beta4Gal-T1
• betaGlcNAc beta
• CDG2D
• EC 2.4.1
• GGTB2
• GGTB2DKFZp686N19253
• glycoprotein-4-beta-galactosyltransferase 2
• GT1
• GTB
• lactose synthase
• MGC50983
• UDP-Gal
• UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1
• UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Background

beta 4GalT1 is one of seven beta 1,4 galactosyltransferases that transfer galactose in a beta 1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. By sequence similarity, the beta 4GalTs form four groups: beta 4GalT1 and beta 4GalT2, beta 4GalT3 and beta 4GalT4, beta 4GalT5 and beta 4GalT6, and beta 4GalT7 (1). beta 4GalT1 is unique among the seven enzymes because it can be expressed either as membrane associated form or secreted form (2). The secreted form is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha -lactalbumin to catalyze the synthesis of lactose (3). The membrane form can reside either in the Golgi apparatus, where it adds galactose to N-acetylglucosamine residues, or on cell surface, where it functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix (4). The two enzymatic forms result from alternate transcription initiation sites and post-translational processing (5). Defects in beta 4GalT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) (6).

        

1. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473:35.
2. Yamaguchi, N and Fukuda, M.N. (1995) J. Biol. Chem. 270:12170.
3. Appert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138:224.
4. Lopez, L.C. et al. (1991) J. Biol. Chem. 266:15984.
5. Mengle-Gaw, L. et al. (1991) Biochem. Biophys. Res. Commun. 176:1269.
6. Hansske, B. et al. (2002) J. Clin. Invest. 109:725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1,4-Galactosyltransferase 1/B4GalT1 Antibody (AF3609)(5)

Publications using AF3609

• Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan Communications Biology 8/1/2022 [PMID: 35915223]
• Laura Hobohm, Tomas Koudelka, Fenja H. Bahr, Jule Truberg, Sebastian Kapell, Sarah-Sophie Schacht et al. N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion Cellular and Molecular Life Sciences 3/13/2022 [PMID: 35279766]
• P Fisher, H Spencer, J Thomas-Oat, AJ Wood, D Ungar Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation Cell Rep, 2019-04-23;27(4):1231-1243.e6. 2019-04-23 [PMID: 31018136] (Western Blot, Human)
• LK Climer, ID Pokrovskay, JB Blackburn, VV Lupashin Membrane detachment is not essential for COG complex function Mol. Biol. Cell, 2018-03-30;0(0):. 2018-03-30 [PMID: 29467253] (Western Blot, Human)
• Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J, 2014-10-29;33(24):2890-905. 2014-10-29 [PMID: 25354954] (Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: B4GALT1
• Uniprot:
  • P15291()