Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody [Unconjugated]

Western blot shows lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human beta -1,4-Glucuronyltransferase 1/B4GAT1
  Asp43-Cys415
  Accession # O43505
• Specificity: Detects human and mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 in Western blot. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: B4GAT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody [Unconjugated]

• B3GNT1
• B3GN-T1
• B4GAT1
• beta-1,3-N-acetylglucosaminyltransferase bGnT-6
• BETA3GNTI
• EC 2.4.1.149
• i-beta-1,3-N-acetylglucosaminyltransferase
• iGAT
• iGNT
• MDDGA13
• N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
• Poly-N-acetyllactosamine extension enzyme
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1B3GNT6
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

Background

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the beta 3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B4GAT1
• Uniprot:
  • O43505(Human)