Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody (724057) [Unconjugated]

Western blot shows lysates of IMR‑32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Monoclonal Antibody (Catalog # MAB6664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clone: 724057
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody (724057) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human beta -1,4-Glucuronyltransferase 1/B4GAT1 Antibody
  Asp43-Cys415
  Accession # O43505
• Specificity: Detects human beta -1,4-Glucuronyltransferase 1/B4GAT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B3GNT2, rhB3GNT6, or rhB4GalT1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: B4GAT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody (724057) [Unconjugated]

• B3GNT1
• B3GN-T1
• B4GAT1
• beta-1,3-N-acetylglucosaminyltransferase bGnT-6
• BETA3GNTI
• EC 2.4.1.149
• i-beta-1,3-N-acetylglucosaminyltransferase
• iGAT
• iGNT
• MDDGA13
• N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
• Poly-N-acetyllactosamine extension enzyme
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1B3GNT6
• UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6

Background

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beta-1,3-N-Acetylglucosaminyltransferase 1/B3GNT1 Antibody (MAB6664)(1)

Publication using MAB6664

• Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J, 2014;33(24):2890-905. 2014 [PMID: 25354954] (Western Blot, Human)

Bioinformatics

• Gene Symbol: B4GAT1
• Uniprot:
  • O43505(Human)