Human BACE-1 ELISA Kit (Chemiluminescence)

ELISA: Human BACE-1 ELISA Kit (Chemiluminescence) [NBP2-66742] - Samples were spiked with high concentrations of Human BACE-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human BACE-1 ELISA Kit (Chemiluminescence) [NBP2-66742] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 ng/mL

Human BACE-1 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human BACE1 in samples. No significant cross-reactivity or interference between Human BACE1 and analogues was observed.
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 10.2%
• Intra-Assay: CV% < 8.52%
• Spike Recovery: 84-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: BACE1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human BACE-1 ELISA Kit (Chemiluminescence)

• APP beta-secretase
• ASP2
• Aspartyl protease 2
• BACE1
• BACE-1
• BACEAsp 2
• beta-secretase 1 precursor variant 1
• beta-secretase 1
• beta-site amyloid beta A4 precursor protein-cleaving enzyme
• Beta-site amyloid precursor protein cleaving enzyme 1
• Beta-site APP cleaving enzyme 1
• beta-site APP-cleaving enzyme 1
• beta-site APP-cleaving enzyme
• EC 3.4.23
• EC 3.4.23.46
• FLJ90568
• HSPC104
• KIAA1149
• memapsin-2
• Membrane-associated aspartic protease 2
• transmembrane aspartic proteinase Asp2

Background

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for BACE-1 ELISA Kit (NBP2-66742). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BACE1