B7-H7/HHLA2 Products

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank">

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">

8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/ HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." class="big_lightbox" target="_blank">

8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 µg/mL) (blue line). Binding is completely blocked (red) by 50 µg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 isotype (Catalog # MAB002) and irrelevant mouse monoclonal antibody were used as controls and did not affect TMIGD2/ CD28H binding to B7-H7/HHLA2 (not shown)." class="big_lightbox" target="_blank">

8316-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Cynomolgus B7-H7/HHLA2 (Catalog # 10109-B7) binds with an ED₅₀ of 0.2-1.2 µg/mL" class="big_lightbox" target="_blank">

8316-TR) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL." class="big_lightbox" target="_blank">

2 μg/lane of Recombinant Human B7-H7/HHLA2 His-tag Protein (Catalog # 10475-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver stain, showing bands at 55-65 kDa." class="big_lightbox" target="_blank">