ATP5E Antibody [DyLight 650]

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Product Details

Summary
Reactivity Dr, Hu(-)Species Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

ATP5E Antibody [DyLight 650] Summary

Immunogen
Synthetic peptide made to a portion of Drosophila ATP5E (within residues 1-50). [Swiss-Prot# Q9VXN2]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5E
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This ATP5E antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis where a band can be seen at ~7 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Drosophila. Does not react with human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATP5E Antibody [DyLight 650]

  • ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
  • ATPE
  • F(0)F(1)-ATPase
  • H(+)-transporting two-sector ATPase
  • MGC104243
  • mitochondrial ATP synthase epsilon chain
  • mitochondrial ATPase
  • mitochondrial

Background

The Drosophila gene for the ATP synthase epsilon subunit (ATP5E) is known as stunted or sun (Kidd et al. 2005). Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATP5E