ATP5E Antibody [HRP]

Product Discontinued

ATP5E Antibody [HRP] Summary

• Immunogen: Synthetic peptide made to a sequence between amino acids 2-51 of human ATP5E. [Swiss-Prot# P56381]
• Localization: Mitochondrion. Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This ATP5E antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis where a band can be seen at ~7 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Human, mouse, rat, porcine, bovine and Xenopus. Does not react with Drosophila.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for ATP5E Antibody [HRP]

• ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
• ATPE
• F(0)F(1)-ATPase
• H(+)-transporting two-sector ATPase
• MGC104243
• mitochondrial ATP synthase epsilon chain
• mitochondrial ATPase
• mitochondrial

Background

ATP5E encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5E encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5E