Recombinant Human Aspartate beta hydroxylase His Protein

SDS-Page: Recombinant Human Aspartate beta hydroxylase Protein [NBP1-40403] - ASPH, 24.5 kDa (217aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Aspartate beta hydroxylase His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 75-270 of Human Aspartate beta hydroxylase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ASPH
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Aspartate beta hydroxylase His Protein

• AAH
• ASP beta-hydroxylase
• aspartate beta-hydroxylaseA beta H-J-J
• aspartyl/asparaginyl-beta-hydroxylase
• BAH
• cardiac junctin
• CASQ2BP1
• EC 1.14.11.16
• HAAHaspartyl/asparaginyl beta-hydroxylase
• humbug
• JCTN
• junctate
• junctin
• Peptide-aspartate beta-dioxygenase

Background

ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ASPH