ARSJ Overexpression Lysate (Native)

Images

 
Western Blot: ARSJ Overexpression Lysate (Adult Normal) [NBL1-07742] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARSJ.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ARSJ Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215725 and based on accession number NM_024590. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens arylsulfatase family, member J (ARSJ), mRNA.
Gene
ARSJ

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
67.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ARSJ

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ARSJ Overexpression Lysate (Native)

  • arylsulfatase family, member J
  • arylsulfatase J
  • ASJ
  • EC 3.1.6
  • EC 3.1.6.-
  • EC 3.1.6.12
  • FLJ23548

Background

Sulfatases (EC 3.1.5.6), such as ARSJ, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00347527-B01P
Species: Hu
Applications: WB, ELISA
NBP1-84209
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86892
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87581
Species: Hu
Applications: IHC, IHC-P
NBP1-83905
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-83678
Species: Hu
Applications: IHC, IHC-P
NBP1-47974
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
AF1970
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-88561
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90306
Species: Hu
Applications: WB, IHC, IHC-P
NB100-74480
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00010926-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
AF4600
Species: Mu
Applications: WB, IP
NBP1-33177
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46476
Species: Hu
Applications: WB, IHC
NBP1-47602
Species: Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
MAB38461
Species: Hu
Applications: WB, IHC

Publications for ARSJ Lysate (NBL1-07742) (0)

There are no publications for ARSJ Lysate (NBL1-07742).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ARSJ Lysate (NBL1-07742) (0)

There are no reviews for ARSJ Lysate (NBL1-07742). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARSJ Lysate (NBL1-07742) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ARSJ Products

Bioinformatics Tool for ARSJ Lysate (NBL1-07742)

Discover related pathways, diseases and genes to ARSJ Lysate (NBL1-07742). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ARSJ Lysate (NBL1-07742)

Discover more about diseases related to ARSJ Lysate (NBL1-07742).

Research Areas for ARSJ Lysate (NBL1-07742)

Find related products by research area.

Blogs on ARSJ

There are no specific blogs for ARSJ, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ARSJ Antibody
NBP2-48658

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARSJ Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ARSJ