Recombinant Human ARF1 Protein

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SDS-Page: Recombinant Human ARF1 Protein [NBP1-45297] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human ARF1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-181 of Human ARF1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ARF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human ARF1 Protein

  • ADP-ribosylation factor 1

Background

ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Blogs on ARF1.

Arf1: A New Focus In Cancer Drug Therapy
ARF1 (ADP-ribosylation factor 1) is a protein in the ARF gene family that is responsible for vesicular trafficking within the cell through its activation of phospholipase D. It is found in the cells golgi apparatus and its main function is intra-Golgi...  Read full blog post.

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Bioinformatics

Gene Symbol ARF1