ARF1 Antibody (SD2002)

Western Blot: ARF1 Antibody (SD2002) [NBP2-67739] - Analysis of ARF1 on NIH/3T3 cells lysates using anti-ARF1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: ARF1 Antibody (SD2002) [NBP2-67739] - Analysis of paraffin-embedded human colon cancer tissue using anti-ARF1 antibody. Counter stained with hematoxylin.

Flow Cytometry: ARF1 Antibody (SD2002) [NBP2-67739] - Analysis of Hela cells with ARF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunohistochemistry-Paraffin: ARF1 Antibody (SD2002) [NBP2-67739] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ARF1 antibody. Counter stained with hematoxylin.

Product Discontinued

ARF1 Antibody (SD2002) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ARF1aa 61-110 / 181. (SwissProt: P84077 Human; SwissProt: P84078 Mouse; SwissProt: P84079 Rat)
• Localization: Golgi apparatus, Cytoplasm, Cell junction , Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ARF1 Antibody (SD2002)

• ADP-ribosylation factor 1
• ARF1

Background

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARF1