APOBEC3G Antibody

Western Blot: APOBEC3G Antibody [NBP1-52509] - NBP1-52509 (0.5µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-52509] - Analysis of anti-APOBEC3G antibody with human kidney at concentration 2.5 ug/ml.

Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-52509] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Product Discontinued

APOBEC3G Antibody Summary

• Immunogen: Peptide with sequence C-DEHSQDLSGRLR, from the C Terminus of the APOBEC3G protein sequence according to NP_068594.1.
• Predicted Species: Monkey (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOBEC3G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA 1:16000
  • Western Blot 0.5-1.5 ug/ml
• Application Notes: Western Blot: Approx. 45kD band observed in lysates of cell line Daudi (calculated MW of 46.4kD according to NP_068594.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Baboon (100%), Chimpanzee (83%), Gorilla (83%), Gibbon (83%), Marmoset (83%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for APOBEC3G Antibody

• APOBEC-related cytidine deaminase
• APOBEC-related protein 9
• APOBEC-related protein
• apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• ARCD
• ARP-9
• bK150C2.7
• CEM-15
• CEM15ARP9
• dJ494G10.1
• DNA dC->dU-editing enzyme APOBEC-3G
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.5
• FLJ12740DNA dC->dU editing enzyme
• MDS019
• phorbolin-like protein MDS019

Background

Anti-APOBEC3G is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOBEC3G
• Entrez:
  • 200316(Human)
• Uniprot:
  • Q9HC16(Human)