Apc1 Antibody

Product Discontinued

Apc1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human APC1. The immunogen region is: Internal.
• Specificity: APC1 (Ab-688) Antibody detects endogenous levels of total APC1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apc1 Antibody

• anaphase promoting complex subunit 1
• anaphase-promoting complex 1 (meiotic checkpoint regulator)
• anaphase-promoting complex subunit 1
• APC1Testis-specific gene 24 protein
• Cyclosome subunit 1
• Mitotic checkpoint regulator
• TSG24MCPR

Background

APC1 (anaphase-promoting complex subunit 1) is a meiotic checkpoint regulator that is a member of the E3 enzyme family. This protein contains Rpn1/2 repeats and has a molecular weight of approximately 216 kD. The APC1 protein is located in the nucleus during interphase, and at the centrosome during metaphase/anaphase. This protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC1 protein functions as a probable scaffold for complex assembly, a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC1 protein is phosphorylated by Cdk1-cyclin B and can also be ubiquitinated and targeted for degradation. The Poly6017 antibody has been shown to be useful for Western blotting of the human APC1 protein. Anaphase promoting complex composed of eight protein subunits APC1-8, APC9-11, Cdc26, Mnd2, and Swm1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC1
• Uniprot:
  • Q9H1A4(Human)